The result showed the presence of Aspergillus niger, Rhizopus nigricans, Mucor mucedo, and Fusarium oxysporum. as responsible for the soft rots of orange fruits in Nigeria. The quantities of the appropriate medium or base medium were weighed. We … Background and Objective: Fruits are subjected to natural contamination by many different kinds of micro-organisms, including pathogens such as fungi. 2019 Jun 13;10:769. doi: 10.3389/fpls.2019.00769. Isolation and Characterisation of Microorganisms Associated with Rot Diseases of Fruit, Stem and Leaf of Carica papaya L. A Project Report Submitted to the Department of Biological Sciences, College of Science and Technology, Covenant University, Canaanland, Ota, Ogun state, Nigeria. 2020 Jun 25;27:e00496. and Rhizopus sp. However, the value obtained for the prevalence of A. niger which caused a disease called black mold on certain fruits and produced potent mycotoxins called ochratoxins that can be harmful to human beings and animals, was higher than the one reported by Mailafia et al.18 who reported the highest occurrence of 38%. The culture media were prepared according to manufacturer’s recommendations. Isolation, Characterization and Identification of Thiram-degrading Microorganisms from Soil Enrichment Cultures Nurettin ŞAHİN Muğla University, Faculty of Arts and Sciences, Department of Biology, ... biochemical tests fungi were identified as Aspergillus niger, A. flavusand Penicilium steckii. In conclusion Eighty two sps of fungi were isolated from undisturbed soils of Mattavara forest, in which Cladosporium, Penicillium and Aspergillus, Fusarium, Trichodermasps are the most commonly occurring fungi due to heavy sporulation and production of secondary metabolites, which prevent the growth of other fungal sps. The post-harvest fruit rots of tomato (Lycopersicum esculentum) in Nigeria. Isolation and Identification o f Fungal Species . Materials and Methods: One hundred fruits comprising of 70 spoilt ones and 30 healthy ones were collected from the market and transported to the laboratory. On the basis of tolerance to endosulfan, an isolate, identified as Aspergillus niger was selected for further The samples were kept in the refrigerator at 4°C before laboratory mycological analysis. Get the latest public health information from CDC: https://www.coronavirus.gov. The inoculated samples and the control were placed in sterile polythene bags and incubated at 28°C for 14 days. However, despite such tremendous benefits of fruits, their existence and life span is threatened by several factors among which are: Climate change, pests, inadequate rainfall and fungal attack. 1979;23(2):105-9. doi: 10.1002/food.19790230202. isolated from soil This research was aimed to isolate cellulolytic fungi showing hyper-cellulase activity. Care should be taken during handling of these fruits and improved technology based preservation methods are suggested to enhance the keeping quality of fruits. Soil samples collected were taken to the laboratory for isolation of Aspergillus niger using potato dextrose agar (PDA). Methodology: Eight different mechanic workshops within Bida were selected and soil samples were collected from each site. The study collected soil samples from three (3) different mechanic workshops soil. The Aspergillus niger has grown well at 30 °C and produced maximum amount of citric acid (80.68 g l-1) followed by 20 °C with 74.21 g l-1 of citric acid (Fig. The mineral portion of soil results from the actions of Weathering and erosion on rock (Dannis, 2012). Morphological characteristics remain the primary tool for detection and identification of Asper-gillus species. In: Prasad D, editor. However, all the bacterial were present in soil amended with layer chicken droppings 5% and 10% throughout the period of bioremediation and absent all throughout the contaminated soil sample plus 2% formaline solution. was the least common. ecosystem particularly soil, often with undesirable effects. USA.gov. The Petri dishes were labelled appropriately and stored in the refrigerator for later use. and Mucor spp. | Production of cellulase enzyme by Aspergillus niger, Aspergillus terreus and Penicillium sp. Over the years, there has been an increase in the need to identify and isolate the fungi associated with their spoilage. 2018;8(6):512‒515. The fungal isolates were sub-cultured on to PDA slants to obtain pure isolates. Pathogenicity test: The Pathogenicity test was represented in Table 3. species; Aspergillus flavus and Rhizopus stolonifer in tomatoes. Isolation of fungi from spoilt fruits: Isolation of the mycological flora followed the method of Dashwood et al.9 and Balali et al.10. This site needs JavaScript to work properly. Even during refrigeration some microbes such as moulds and other fungi produce mycotoxins of various types that are harmful to the consumers4. frequency of occurrence; fruits; fungi; pathogenic; prevalence. Isolation and screening of a hyper producer strain plays a key role in the production of enzyme (Haq et al., 2002b). Sample suspension was prepared by adding 1.0 gm of soil sample to 100 mL distilled water and shaking for 15 min in an orbital shaker. Ilahy R, Tlili I, Siddiqui MW, Hdider C, Lenucci MS. Front Plant Sci. Aspergillus niger had the highest occurrence in pineapple, watermelon, oranges, pawpaw, and tomatoes with a frequency of 38%. The soil fungi were isolated by the soil dilution Isolated Fungi 1 Aspergillus sp. The media were cotton plugged and wrapped with aluminium foil and were autoclaved at 121°C at a pressure of 15 Ibf/in2 for 15 min. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Potential cellulase-producing fungi were isolated from different soil … and Rhizopus spp. Abel-Mallek et al.,1995 also reported preponderant of Aspergillus niger in healthy tomato fruits collected from markets in Assiut, Egypt. ISOLATION OF ASPERGILLUS NIGER The sample was isolated from site in Malaysia which at Bukit tinggi, Pahang.This place have a maximum average temperature of about 38°C and a minimum average temperature of 16°C. RESULTS: Aspergillus niger had the highest occurrence in pineapple, watermelon, oranges, pawpaw, and tomatoes with a frequency of 38%. (Aspergillus niger), B. 2019 Nov 30;24(1):68. doi: 10.1186/s12199-019-0825-5. The pathogenicity tests showed that the fungal isolates affected all the fruits but information is still needed to compare the effect of the fungal isolates on sugar and nutritive content of the fruits. Ikhiwili O.M. (Aspergillus japonicus), C. (Aspergillus flavus), Studies on fungi associated with tomato fruits and effectiveness of some commercial fungicides against three pathogens. Fusarium avenaceum followed with the frequency of occurrence of 31% in fruits such as pineapple, watermelon, oranges, pawpaw, and tomatoes while Penicillium digitatum and Rhizopus stolonifer had the least frequency of 4% each in tomato; and orange and tomato, respectively. genes. eCollection 2020 Sep. Environ Health Prev Med. This study revealed that PSW could serve as a suitable substrate for the bioflocculant producing microorganism- Aspergillus niger (A18) isolated from soil sample. This was followed by suspending the weighed amount of the media in 400 mL of distilled water. Alternaria sp. Isolation, identification, and characterization of an Aspergillus niger bioflocculant-producing strain using potato starch wastewater as nutrilite and its application PLoS One . Ten grams of contaminated soil was added to 100mL of the enrichment medium and was incubated at 30oC for 4 days. These fungal species were confirmed to be causative agents of the spoilage. This research was carried out to investigate the types of fungal flora associated with the spoilage of fruits at Utako market, Abuja, Nigeria. The strains of Aspergillus niger can be isolated from soil, water and air (Wu et al., 2000). Conclusion: In all, 16 fungal isolates were obtained by enrichment of soil samples that had seems exposed to endosulfan before. The molten agar media were allowed to cool to 45°C and the pH adjusted according to the manufacturer’s recommendation. Isolation and identification of Aspergillus niger Aspergillus niger was isolated from soil by serial dilution technique. Get the latest research from NIH: https://www.nih.gov/coronavirus. Various colonies observed in the plates were distinguished on the basis of their cultural characteristics such as colony size, shape, colour, consistency and haemolytic characteristics as described by Fawole and Oso11. Vet World. during the isolation process and dominated with flavusAspergillus from both the maize and soil. Among fifteen fungal isolates studied from these five zones Aspergillus parvisclerotigenus was potent for pectinase production next to Aspergillus niger in form of halozone of 0.6 mm. The inoculated plates were incubated at ambient room temperature (25°C) for 7 days. However, the pathenogenicity of Rhizopus stolonifer was higher than that of the remaining fungal species isolated, while that of Alternaria sp. It’s 28S rDNA sequence also had some significant identity (>90%) with different subspecies of Aspergillus. and Aspergilus fumigatus among the spoilt fruits indicated the degree of pathenogenicity conferred by the family Aspergillaceae in inducing fruits spoilage. 2018 Jan 5;13(1):e0190236. Aspergillus fumigatus and Mucor species Public health risks related to food safety issues in the food market: a systematic literature review. Results: The infected fruits were surface sterilized with cotton wool soaked in 0.1% mercury chloride (HgCl) for 2 min then rinsed 3 times with distilled water. ... contact with soil, dust and water and by poor handling or during postharvest processing. Agar disc containing 1 week old fungal culture were aseptically placed in these holes, then covered and sealed off by means of petroleum jelly. Thin sections of the rotten fruits were obtained using a sterile blade and inoculated onto PDA media and incubation was carried out at 27°C for 5 days. Several studies have also reported that Aspergillus spp. Nahrung. Healthy samples were surface sterilized with 0.1% HgCl.
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